Effects of amino acid-supplemented diets on sister chromatid exchange frequency of rats treated with cyclophosphamide.

Abstract

Less balanced diets are known to induce higher sister chromatid exchange (SCE) frequency in rats. The SCE frequency of various diet rat groups correlated well with the degree of variation among the fasting plasma profile equivalent (FPPE) values of amino acids in their diets. To narrow the differences among the FPPE values of essential amino acids, three limiting amino acids, lysine, tryptophan, threonine, were added to the regular diet (Diet P) and to a less balanced diet, Diet G. The amounts of amino acid supplemented were calculated based on the FPPE concept. It was found that the addition of the three amino acids could reduce SCE frequency of those rats treated with a mutagen, cyclophosphamide (CP), especially at a higher dosage of CP.