Abstract
Barley has two nitrate reductase (NR) genes; one encoding the NADH-specific NR and the other encoding the NAD(P)H-bispecific NR. We examined the effects of exogenously supplied amides on the nitrate-induced expression of two NR genes in hydroponic cultured barley seedlings. In leaves and roots of barley seedlings pretreated with glutamine and asparagine and subsequently treated with nitrate, NR mRNAs were transiently accumulated and then rapidly declined. Total nitrate uptake was inhibited only 25 to 30% by treatment of amides indicating that the repression of NR gene expression by amides was not caused by the inhibition of nitrate uptake. Concentration of tissue amides were increased by externally supplied amides and well correlated with the levels of NR mRNAs in leaves and roots. These results suggested that amides act as a factor controlling NR gene expression in barley.
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