Abstract
Abstract 1. 1. Rat-liver phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxylyase (transphosphorylating), EC 4.1.1.32) levels have been measured following various hormonal treatments and fasting, and in control animals. 2. 2. Adrenalectomy and hydrocortisone administration were without significant effect on mitochondrial carboxykinase levels, although amounts of this enzyme in soluble fraction of these same livers were nearly doubled by administration of the glucocorticoid. 3. 3. Alloxan diabetes, insulin administration, and fasting also were without statistically significant effect on levels of mitochondrial carboxykinase expressed per mg protein; increases of 55% and 51% respectively, in mitochondrial activity per g liver or per 100 g body weight were noted in diabetic animals. 4. 4. It is conluded that any concerted participation of rat-liver pyruvate carboxylase (pyruvate: CO2 ligase (ADP), EC 6.4.1.1) plus phosphoenolpyruvate carboxykinase in glucogenesis must involve principally the extramitochondrial carboxykinase.
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