Effects of altered coronary perfusion pressure on function and metabolism of normal and cardiomyopathic hamster hearts

Abstract

The effects of a sudden decrease in coronary perfusion pressure from 140 to 0 cmH2O for a 10-second interval were analyzed in normal and cardiomyopathic hamster hearts to determine whether cardiomyopathy would affect the relationship between altered coronary perfusion pressure and left ventricular geometry, wall thickness, myocardial hydrodynamics, and hemodynamics. In normal hamsters, an acute reduction in coronary perfusion pressure resulted in a decrease in left ventricular short axis epicardial cross-sectional area, base to apex length, diastolic wall thickness, myocardial water content and developed pressure. In cardiomyopathic hamsters all results induced by lowering the hydrostatic pressure of the perfusing medium were the same except that diastolic wall thickness failed to decline, indicating a decrease in intramyocardial elasticity in dilated cardiomyopathy. In parallel studies, hearts were freeze clamped at end-diastole and high energy phosphates and energy metabolites analyzed. In both normal and cardiomyopathic hamsters no significant changes were observed in ATP, PCr, or Pi levels at 10 s following the decrease in perfusion pressure. However, during the abrupt decrease in coronary perfusion pressure adenosine increased and cAMP decreased in both groups of animals. The erectile effect of altered coronary perfusion pressure is partially attenuated in the cardiomyopathic hamster in which no change in diastolic wall thickness occurs during an abrupt change in the hydrodynamics of the heart.