Effects of allyl sulfur compounds and garlic extract on the expression of Bcl-2, Bax, and p53 in non small cell lung cancer cell lines.

Young-Sook Hong,Ji-Hyung Choi,Yoon-Ae Ham,Jhingook Kim

doi:10.1038/emm.2000.22

Abstract

Allyl sulfur compounds play a major role in the chemoprevention against carcinogenesis. The present study compared the antiproliferative effects of diallyl sulfide (DAS), diallyl disulfide (DADS) and garlic extract on p53-wild type H460 and p53-null type H1299 non small cell lung cancer cells (NSCLC). The DAS and DADS treatment of both H460 and H1299 cells resulted in the highest numbers of cells in apoptotic state as measured by acridine orange staining, however, garlic extract treatment did not induce any significant apoptotic cells by MTT assay. DADS was found to be more effective in inducing apoptosis on NSCLC. The level of p53 protein in H460 cell was increased following DADS treatment. DAS and garlic extract treatment of H460 cells induced a rise in the level of Bax and a fall of Bcl-2 level. These results demonstrate that DAS, DADS and garlic extract are effective in reduction of anti-proliferative gene in NSCLC and suggest that modulation of apoptosis-associated cellular proteins by DAS, DADS and garlic extract may be the mechanism for apoptosis which merit further investigation as potential chemoprevention agents.

Highlights

Epidemiological investigations provide strong evidence that environmental factors are modifiers in the occurrence of some types of human cancer (Wynder and Gori, 1977; Parmer and Bakshi, 1983)
These results demonstrate that diallyl sulfide (DAS), diallyl disulfide (DADS) and garlic extract are effective in reduction of anti-proliperative gene in non small cell lung cancer cells (NSCLC) and suggest that modulation of apoptosis-associated cellular proteins by DAS, DADS and garlic extract may be the mechanism for apoptosis which merit further investigation as potential chemoprevention agents
In order to examine the antiproliferative effects of the organoallylsulfur compounds and garlic extract on each cell line, cells were treated with various concentration of DAS, DADS and garlic extract for 1 h and replaced with new medium

Summary

Introduction

Epidemiological investigations provide strong evidence that environmental factors are modifiers in the occurrence of some types of human cancer (Wynder and Gori, 1977; Parmer and Bakshi, 1983). Recent studies suggested that garlic and related allium foods might protect human from some types of cancer (Steinmetz et al, 1994), the protective effect by these foods was often inconsistent (Dorant et al, 1995). The reason for these inconsistencies remains to be clarified. In spite of numerous studies on the garlic oil component, a study on the whole water soluble garlic extract has never been reported

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Conclusion