Effects of alimentary reconstruction on gastrointestinal hormones in a dog model undergoing subtotal gastrectomy

Abstract

Objective To evaluate effects of alimentary reconstruction procedures （integral continual jejunal interposition, Billroth Ⅱ and isolated jejunal interposition） after subtotal gastrectomy on postoperative plasma gastrin, motilin and cholecystokinin. Methods Twenty-four dogs were divided into 3 groups undergoing distal subtotal gastrectomy and three different digestive tract reconstruction （integral continual jejunal interposition, Billroth Ⅱ and isolated jejunal interposition）. The concentration of plasma gastrin, motilin and cholecystokinin were detected by enzyme-linked immunosorbent assay before and after operation. Results Two months after operation, plasma gastrin level of the integral continual jejunal interposition group （2. 2 ±0. 7 ） ng/L, （ 3.9 ± 0. 8 ） ng/L was significantly lower than that of preoperative both in fasting and postprandial state （3.8 ± 1.0） ng/L, （5.3 ± 1.6） ng/L, all P 〈0.05, but was significantly higher than other two groups in postprandial state （2. 7 ± 1.0） ng/L, （3.6 ±0. 6） ng/L, P 〈0. 05. Two months after operation, plasma motilin concentration of integral continual jejunal interposition group （577 ±204） ng/L, （1003 ± 209） ng/L were significantly higher than that of preoperative both in fasting and postprandial （429 ± 128） ng/L, （854 ± 218 ） ng/L, P 〈 0. 05. The postoperative plasma motilin of integral continual jejunal interposition group （ 1003 ± 209 ） ng/L was significantly higher than other two groups in postprandial state （840 ±205） ng/L, （986 ± 189） ng/L, P 〈0. 05. Two months after operation,plasma cholecystokinin concentration of integral continual jejunal interposition group （ 19.6 ± 2.0 ） ng/Lwere significantly higher than that of preoperative both in postprandial （ 19.0 ± 2. 0） ng/L, P 〈 0. 05. The postoperative plasma cholecystokinin of integral continual jejunal interposition group （ 19. 6 ± 2. 0） ng/L was significantly lower than other two groups （22.2 ± 2. 1 ） ng/L, （20. 1 ± 2. 5 ） ng/L, P 〈 0. 05. Conclusion Integral continual jejunal interposition after distal gastrectomy maintains the postoperative plasma motilin and gastrin in a relatively higher level and decreases the concentration of plasma cholecystokinin. Key words: Gastroenterostomy ; Gastrins ; Cholecystokinin ; Disease modals, animal