Abstract
To study the influence of albumin on changes of liver function in the extrahepatic biliary obstruction through an experimental model in rats. Sixty rats were divided into four groups: Group C (Control): 6 animals. Group M (Fictitious Operation): 18 rats underwent laparotomy and handling of the bile ducts; Groups O (extrahepatic biliary obstruction) and A (Treated with 2% albumin): 18 animals in each group underwent ligation of the ductus liver; The animals in groups M, O and A were divided into three subgroups of 6 animals each to be killed in the 7, 14 and 21 days postoperative (POD). Blood was drawn for determination of total bilirubin (TB), indirect bilirubin (IB), direct bilirubin (DB), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). On POD 7, BI levels were 4.5 mg / dl in group O and 2.1 mg / dl in group A (p = 0.025). On the 14th POD, the levels of PA were 1185.2 U / l in the group and O 458.3 U / l in group A (p = 0.004). ALT levels were 101.7 U / l in the group O and 75.7 U / l in group A (= 0.037). On POD 21, the levels of ALP were 1069.5 U / l in the group O and 468.3 U / l in group A (p = 0, 004). The administration of albumin reduced the serum levels of bilirubin in the 7th day of supplementation.
Highlights
The extrahepatic biliary obstruction is associated with high rates of postoperative morbidity and mortality
Hepatic changes may be evidenced by increased serum levels of direct bilirubin, resulting from canalicular backscatter, increased levels of alkaline phosphatase (ALP), gamma-glutamyltranspeptidase, while levels of alanine and aspartate transaminase (AST and ALT) can be found in normal or high, all due to changes in hepatocyte membrane secondary to bile stasis[3,4]
Group A: 06 animals underwent laparotomy with ligation of hepatic duct who were treated with albumin 2% ad libitum and divided into A7, A14 and A21, which will be sacrificed at 21 days and 7.14 after manipulation, for 21 days
Summary
The extrahepatic biliary obstruction is associated with high rates of postoperative morbidity and mortality. Regardless of origin, it is associated with morphological and functional damage of the liver[1,2], and a lower immune resistance. The colloid osmotic pressure of interstitial fluid that “bathes” the hepatocyte is the most important regulator of its production[5]. Their production depends on food intake and fasting, the diet deficient in protein, leads to a drastic reduction in their serum[5]. Its functions are: the maintenance of colloid osmotic pressure, the carrying of substances, anticoagulation role in the metabolism of endogenous substances and interest in the maintenance of plasma pH and microvascular integrity[5,6]
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