Effects of albendazole, artesunate, praziquantel and miltefosine, on Opisthorchis viverrini cercariae and mature metacercariae.

Abstract

To explore larvicidal effects of anthelmintic drugs on Opisthorchis viverrini (O.viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. The larvicidal activities of albendazole (Al), artesunate (Ar), praziquantel (Pzq) and miltefosine (Mf) on O.viverrini cercariae and mature metacercariae were investigated. Lethal concentrations (LC50 and LC95) of these drugs were determined. Mature metacercariae previously exposed to various concentrations of the drugs were administered to hamsters. Worms were harvested 30d post infection and worm recovery rates calculated. Al, Ar, Pzq and Mf produced morphological degeneration and induced shedding tails of cercariae after 24h exposure. The LC50 and LC95 of Al, Ar, Pzq and Mf on cercariae were 0.720 and 1.139, 0.350 and 0.861, 0.017 and 0.693, and 0.530 and 1.134ppm, respectively. LC50 and LC95 of Ar on mature metacercariae were 303.643 and 446.237ppm and of Mf were 289.711 and 631.781ppm, respectively but no lethal effect in Pzq- and Al-treated groups (up to 1ppt). No worms were found in hamsters administered Pzq-treated metacercariae. The adult worms from Al-treated metacercariae were significantly bigger in size compared to the control group (P<0.05). Fecundity and body width were greater in adults from Mf-treated metacercariae compared to the control group (P<0.05). The larvicidal effects of these drugs were high efficacy to O.viverrini cercariae but lesser efficacy to metacercariae. It should be further studied with the eventual aim of developing a field-based control program.