Effects of Akt inhibitor deguelin on the growth of bladder cancer cells

Abstract

Objectives To study the effects of Akt inhibitor deguelin on the growth of human bladder cancer cell lines T-24,and to elucidate the possible mechanism of such effects.Methods MTT method was used to determine growth-inhibitory effect of deguelin on human bladder cancer T-24 cells.The cell cycle was detected by flow cytometry (FCM).Apoptosis induced by deguelin in T-24 cells was determined using DNA fragmentation assay.The expression of MDM2 and GSK3β in mRNA and protein levels were detected by RT-PCR and Western blotting respectively.Results The deguelin singnificantly inhibited cell proliferation,blocked the cell cycle in the G0/G1 phase and induced apoptosis in T-24 cells.The expression levels of MDM2 mRNA and protein were down-regulated,while the expression levels of GSK3 β mRNA and proteins were up-regulated when T-24 cell were treated by deguelin.Conclusions Deguelin can inhibit the proliferation and induce the apoptosis in T-24 human bladder cancer cells.These effects may be related with its function in down-regulating MDM2 and up-regulating GSK3 β,both of them are down-stream signal meleculars in the AKt pathway. Key words: Urinary Bladder Neoplasms; Derris