Abstract

In the previous two studies it was shown that, beginning with maturation, definite progressive morphologic and physiologic changes took place in the egg, both within and without the body, such as (i) an increase in volume of the egg, (2) a loss of sur rounding jelly layer, (@) a retardation in the rate and change in the manner of forming the fertilization membrane, (@) a retarda tion and inhibition in cleavage. These changes served as measur able and corroborative evidences of the physiologic condition or vitality of the eggs of a female at any time, and served to measure the deterioration or loss of vitality with age. There were other symptoms or evidences of physiologic de terioration, especially in late stages of ageing, or overripening, which will be discussed briefly in this paper. These changes include: (i) Agglutination of eggs, (2) fusion of eggs, (3) abnormal cleavage, (4) separation of blastomeres and (@) cytolysis of the eggs. The same technique, the same nomenclature, the same pre cautions and the same three species of sea urchin were used as in Parts I. and II. For details of these I must refer to these studies.

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