Effects of ageing and gender on contractile properties in human skeletal muscle and single fibres

Abstract

The objective of this study is to improve our understanding of the mechanisms underlying the ageing- and gender-related muscle weakness. Ageing- and gender-related differences in regulation of muscle contraction have been studied in knee-extensor muscles at the whole muscle and single muscle fibre levels in young and old sedentary men and women. In vivo knee-extensor muscle function was measured at slow (30 degrees s(-1)) and faster (180 degrees s(-1)) speeds of movement. Maximum velocity of unloaded shortening (V(0)) and maximum force normalized to cross-sectional area (CSA) [specific tension (ST)] were measured in single 'skinned' skeletal muscle fibre segments. Significant ageing- and gender-related differences were observed in muscle torque. A 33-55% ageing-related decline (P < 0.001) in maximum torque was observed irrespective of gender. At the single muscle fibre level, the ageing-related decline in knee-extensor muscle function was accompanied by a 20-28% decline in ST in muscle fibres expressing the type I MyHC isoform in both men and women, and a 29% decline in type IIa muscle fibre CSA, but the decreased fast-twitch fibre size was restricted to the men. Furthermore, in both men and women, V(0) decreased in muscle cells expressing the type I and IIa MyHC isoforms. The present results provide evidence of specific ageing- and gender-related differences in regulation of muscle contraction at the cellular level. It is suggested that these cellular changes have a significant impact on muscle function and the ageing-related motor handicap.