Effects of age and clustered hypoxia on [ 125I]substance P binding to neurotachykinin-1 receptors in brainstem of developing swine

Abstract

This work focused on the postnatal development of substance P-bound neurotachykinin-1 (NK-1) receptors in the porcine brainstem using 2–3-, 6–11-, 16–18-, and 21–28-day-old piglets versus adult, and on alterations in these receptors after single and six-daily repeated clustered hypoxia using 6–11- and 21–28-day-old piglets. NK-1 receptor localization and densities were determined by quantitative autoradiography using mono-iodinated Bolton-Hunter substance P ([ 125I]BHSP). Slide-mounted brainstem sections, incubated in [ 125I]BHSP and then exposed to film, have shown [ 125I]BHSP binding throughout many brainstem nuclei and tracts, including the ambigual/periambigual (nAmb), dorsal motor vagal (dmnv), gigantocellular (nGC), hypoglossal (nHyp), medial parabrachial (nPBM), lateral reticular (nRL), raphe magnus (nRMg), raphe obscurus (nROb) and solitary tract (nTS) nuclei. NK-1 receptor densities decreased with age. As compared to normoxia, NK-1 receptor densities increased significantly after the six-daily hypoxia protocol in nAmb, dmnv, nHyp, nRL, nRMg, nROb, and nTS of both the young and older age groups. This increase may represent receptor upregulation as an adaptation to repeated hypoxia.