Abstract
Salmonella newbrunswick was aerosolized with distilled water into a duct with temperature and relative humidity control of the air. Airborne bacteria were collected on a membrane filter and held in the airstream for ½ hr. Cells were rehydrated and held in distilled water for ½ hr; then they were centrifuged and the supernatant fluids tested for various inorganic chemicals and organic materials that are essential for normal functions. Spot tests revealed potassium, magnesium, phosphates, simple carbohydrates, and amino acids in the supernatant fluids from the bacteria. Sodium was definitely identified in < 10% of the tests on the fluids. An increase over the unaerosolized control in absorbance of the supernatant fluids at 265nm was noticed. The increase may be attributed to partial destruction of ribosomes observed by electron microscopy.
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