Effects of administered sodium ferulate on the repair of glutamate-induced excitotoxic neuronal impair meat in mouse fetal brain

Abstract

Objective To investigate the effects of administered sodium ferulate (SF) on the repair of glutamate-induced excitotoxic neuronal impairment in mouse fetal brain.Methods Pregnant females were randomly divided into control,SF,MSG,and MSG + SF groups,n=10.The animals in MSG group received ig administration of monosodium glutamate (MSG,1.0,2.0,4.0g/kg,onee) at 17-day.The animals in MSG + SF and SF groups received ig administration of MSG (4.0g/kg,once) or normal saline at 17day,and ip administration of SF (40 mg/kg) starting at 18 dpo,once-daily for 2～3 d;the infant mice from the mothers treated with MSG +SF received ip administration of SF (40 mg/kg) after the birth,once-daily for 20 days.The animals in control and MSG groups received ig or ip administration of normal saline simultaneously,respectively.At the 31 st day after the birth the behavioural tests were performed,and the histopathology of the animal brains was studied.Results At the 31 st day after the birth,the number of MSG-treated mice which could crawl along a rope (5/13) was obviously less than that of control(12/14) (P<0.01) ;at the 52th day after the birth,correct responses of MSG-treated group in Ymaze test (17.1/20) were significantly less than that of control (19.4/20)(P<0.01).Examination of histopathology displayed MSG-induced hippocampal lesions were characterized by intracellular edema,degeneration and necrosis of neurons,and hyperplasia.However,the number of MSG+ SF-treated mice which could crawl along a rope (10/13) was close to that of control (12/14);at the 52th day after the birth,correct responses of Y-maze test in the MSG + SF group (19.1/20) were close those in the control (19.4/20).Examination of histopathology displayed that slight hippocampal lesions appeared in the MSG + SF-treated mice.Conclusion Long-term administration of SF may be feasible in repairing glutamate-induced exeitotoxic neuronal impairment in infant mice. Key words: Excitotoxicity; Sodium ferulate; Brain repair; Infant mice