Abstract
Objective To investigate the effects of adiponectin on apoptosis of airway smooth muscle cells (ASMC) and the related mechanism. Methods ASMC of rats were cultured in vitro. The expression of adiponectin receptors in ASMC was detected by reverse transcription-polymerase chain reaction. The rat ASMC cultured was incubated with different concentrations of adiponectin for 48 hours. Apoptosis rate was detected by flow cytometry. The protein expression of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) was detected by Western blot. Results Adiponectin could promote apoptosis of ASMC, and there was concentration-dependent ( r =0.952, P <0. 01). Western blot indicated that the expression of phosphorylated AMPK increased with the incremental concentrations of adiponectin(r =0. 907, P <0. 01). Conclusions Adiponectin may further activate the apoptotic signaling pathway and induce the apoptosis of ASMC by activating AMPK. Key words: Adiponectin; Airway smooth muscle cell; Apoptosis; Adenosine monophosphate-activated protein kinase
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