Effects of acyl-CoA synthetase 5 silencing by siRNA on expression and proliferation of colon carcinoma cell lines

Abstract

Objective To investigate the effects of acyl-CoA synthetase 5 (ACS5) silencing by siRNA on expression and proliferation of colon carcinoma cell lines. Methods The expression of ACS5 in 30 case colon carcinoma and adjacent tissues were analyzed by immunohistochemical staining. The siRNA of ACS5 with Lipofectamine2000TM was transfected into colon carcinoma cell lines (HT-29 and SW480). The expression of ACS5 in colon carcinoma cell lines (HT-29 and SW480) was detected by real-time reverse transcription polymerase chain reaction. Proliferation of colon carcinoma cell lines was analyzed by 3-(4, 5-dimenthylthiazol-2-yl)-5-(3-carboxymethoxy- phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Results The expression of ACS5 in colon cancer was significantly higher than in adjacent tissues by immunohistochemical staining. The mRNA of ACS5 in siRNA-ACS5 group (0.18±0.03) was significantly lower than in NC siRNA group (2.55±0.31) and blank control group (2.48±0.12) in HT-29 colon cancer lines, and the inhibition ratio was 92.96% (F=146.9, P<0.01). The mRNA of ACS5 in siRNA-ACS5 group (0.14±0.01) was significantly lower than in NC siRNA group (1.21±0.05) and blank control group (1±0.03) in SW480 colon cancer lines, and the inhibition ratio was 88.5% (F=826.5.9, P<0.01). Proliferation of HT-29 and SW480 colon cancer line in siRNA-ACS5 group was slower on 72 h and 96 h than in NC siRNA group and blank control group (P<0.05). Conclusions Expression of ACS5 is elevated in colon cancer tissues. siRNA interference of colon cancer line downregulated ACS5 expression and inhibited the proliferation of the colon cancer cells. Key words: RNA, small interfering; Coenzyme A ligases/ME; Colonic neoplasms/ME/PA