Abstract
SummaryThe aim of this research was to compare hormone‐free medium with media with regulator substances (activated charcoal, cytokinins, polyamine biosynthesis inhibitor and chlorocholine chloride) used for microtuber induction and development. Explants of cvs Monalisa, Primura and Spunta were multiplied subculturing nodal segments on plant growth regulator‐free Murashige & Skoog (1962) (MS) medium. When the plantlets had 6–8 nodes, single‐node stem segments were excised and transferred to eight tuberisation media, each consisting of MS basal components supplemented with sucrose (8% w/v) and various regulator substances. The control was a regulator‐free medium including only sucrose. Results were expressed as the number and weight of microtubers per nodal explant.The cultivars showed wide variations in the mean weight of microtubers, ranging from 44.6 mg (Primura) to 77.5 mg (Spunta), and nearly all plants produced tubers. Medium containing activated charcoal gave the highest rate of tuberisation and the largest microtubers. It thus played a role in optimising conditions for rapid, mass tuberisation of these cultivars, and produced large microtubers for field planting.
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