Abstract
Synchronized Chinese hamster fibroblasts (Don-C) were cultured for 6 h in the presence of amethopterin, 5-fluorodeoxyuridine (FUdR), or actinomycin D and were then examined by electron microscopy of thin sections for the presence or absence of daughter procentrioles. Control cells were at mid S phase and contained obvious procentrioles in about 1 profile out of 140. Treatment with FUdR or amethopterin did not reduce this frequency significantly. However, serial section analysis did demonstrate a lack of procentrioles in some cells treated with amethopterin. Actinomycin was quite effective in preventing daughter procentriole formation.
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