Abstract
The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase being 10–50 fold more sensitive than the (NiFeSe) hydrogenase. In addition the Ni(III) EPR signal (g∼2.3) of the “as isolated” (NiFe) hydrogenase was significantly decreased in intensity upon exposure to acetylene.
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