Abstract
To clarify the effects of a single injection of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on bone, rhG-CSF (100, 1,000, and 5,000 μg/kg) was subcutaneously given to 6-week-old rats, and the femur and tibia were evaluated histopathologically at 2, 4 and 7 days after the injection. A significant increase in WBC counts related to the major pharmacological activity of G-CSF was observed in the rhG-CSF-treated groups at 1 to 2 days after the injection. Bone changes were found only in rats treated with 5,000 μg/kg of rhG-CSF. The development of bone changes lagged behind the increase in WBC counts, and the bone changes occurred at 4 and 7 days after the injection. At 4 days after the injection, accelerated osteoclastic bone resorption was observed in the metaphyseal spongy trabeculae of femur and tibia. At 7 days after the injection, a small amount of newly formed bone due to intramembranous ossification was seen in the metaphysis of femur. These results suggest that the higher dose of rhG-CSF may intrinsically induce bone changes with a particular histopathological nature in rats.
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