Effects of a phospholipid cosurfactant on external coalescence in water-in-oil-in-water double-emulsion globules

Abstract

The activated form of N-glutaryl-phosphatidylethanolamine ( aNGPE) has been incorporated into water-in-oil-in-water (W 1/O/W 2) double-emulsion globules. Preparation of individual globules within a cylindrical capillary and video microscopy of their behavior enabled determination of the rate of coalescence of internal aqueous droplets (W 1) with the external aqueous phase (W 2), termed external coalescence. The presence of Tween 80 in the external phase at concentrations of 0.01 and 0.012 M effected such coalescence. The concentration of Span 80 in the oil phase exceeded that of aNGPE considerably, with the ratio of Span 80 to aNGPE molecules ranging from 60 to 1363. Despite the low relative concentration of aNGPE, external coalescence of the internal droplets was prolonged in globules containing this cosurfactant as compared to globules containing only Span 80. Increasing the amount of phospholipid in the oil phase did not significantly affect the rate of release, indicating that within our range of concentrations (22.0–91.3 μM) there was little variation in the amount of phospholipid adsorbing at the interface. These results indicate that, in addition to its suitable protein-binding functionality, aNGPE can prolong the release of internal droplets in a W 1/O/W 2 globule; its use as a cosurfactant shows potential benefits for drug-delivery applications.