Abstract
An experimental dentifrice containing nano-silver fluoride (NSF) and a sodium fluoride (NaF) toothpaste were tested in vitro, against S. mutans, to evaluate the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), antiadherence, antiacid, enamel microhardness, and OCT. The microdilution technique was used to determine the MIC and MBC. Fragments of deciduous enamel were treated with dentifrice slurries, containing bacterial suspension and PBS-treated saliva. The quantification of the microorganisms that adhered to the enamel was determined after 24 hours of incubation, and media pH readings were performed after 2 hours and 24 hours. Deciduous teeth were evaluated for microhardness and OCT during 14 days of pH cycling. Data were statistically analyzed using Student's t-test, Mann–Whitney U test, ANOVA, and Tukey tests at 5% of significance. Dentifrices containing NSF presented a lower MIC and higher statistically significant results compared to NaF dentifrices with respect to preventing bacterial adhesion and pH decreases. NSF and NaF dentifrices showed the same ability to avoid enamel demineralization corroborated by the OCT images. The NSF formulation had a better antibacterial effect compared to NaF dentifrices and similar action on the demineralization of enamel indicating their potential effectiveness to prevent caries.
Highlights
Mutans streptococci produce short-chain acids which dissolve the hard tissue of teeth. ey are highly acidogenic and metabolize sucrose to synthesize insoluble extracellular polysaccharides which enhance their adherence to the tooth
S. mutans is more cariogenic because it produces cell-surface proteins that help it to adhere to the dental surface [2]
Silver nanoparticle (NSF) dentifrice Sodium fluoride (NaF) dentifrice Deionized water water, and placed into the test tubes containing the bacterial suspension and PBS-treated saliva. e fragments were kept in this fluid for 2 h at 37°C. e fragments were transferred to Eppendorf tubes containing 1 mL of sterile saline (0.9% sodium chloride) and sonicated for 30 sec at 50 W to release the bacteria adhered to the enamel
Summary
Joas Araujo Teixeira, Amitis Vieira Costa e Silva ,1 Valdeci Elias dos Santos Junior ,1 Paulo Correia de Melo Junior, Manuela Arnaud, Maria Goretti Lima, Miguel Angel Pelagio Flores, Thayza Christina Montenegro Stamford, Jose Ricardo Dias Pereira, Andrea Gadelha Ribeiro Targino, Andre Galembeck ,2 and Aronita Rosenblatt. An experimental dentifrice containing nano-silver fluoride (NSF) and a sodium fluoride (NaF) toothpaste were tested in vitro, against S. mutans, to evaluate the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), antiadherence, antiacid, enamel microhardness, and OCT. E microdilution technique was used to determine the MIC and MBC. Fragments of deciduous enamel were treated with dentifrice slurries, containing bacterial suspension and PBS-treated saliva. Dentifrices containing NSF presented a lower MIC and higher statistically significant results compared to NaF dentifrices with respect to preventing bacterial adhesion and pH decreases. NSF and NaF dentifrices showed the same ability to avoid enamel demineralization corroborated by the OCT images. E NSF formulation had a better antibacterial effect compared to NaF dentifrices and similar action on the demineralization of enamel indicating their potential effectiveness to prevent caries NSF and NaF dentifrices showed the same ability to avoid enamel demineralization corroborated by the OCT images. e NSF formulation had a better antibacterial effect compared to NaF dentifrices and similar action on the demineralization of enamel indicating their potential effectiveness to prevent caries
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