Abstract
Aim. To study the effects of a high-fat, high-carbohydrate diet on erythrocytes and platelets of rats.Materials and methods. Male Wistar rats (n = 23) were used for the study. The rats were divided into a control group and an experimental group. The rats from the control group were fed with standard rat chow. The rats from the experimental group had received a high-fat and high-carbohydrate diet for 12 weeks. In the rats, body weight and blood pressure (BP) were measured, an oral glucose tolerance test was carried out, and hematological and lipid metabolism parameters were analyzed. The conductance of erythrocyte KCa-channels was measured by the potentiometric method, and platelet aggregation was determined by the turbidimetric method.Results. Feeding the rats with a high-fat, high-carbohydrate diet for 12 weeks resulted in obesity, BP elevation, hyperglycemia, impaired glucose tolerance, and dyslipidemia with pronounced triglyceridemia. In the experimental group, a rise in the number of leukocytes, mainly due to granulocytes, and an increase in the number of platelets and their collagen-induced aggregation were observed. The red blood cell count in the rats of the experimental group did not significantly differ from that of the control group. In the experimental group, multidirectional changes in the membrane potential were observed in response to the stimulation of the KCa-channels in the erythrocyte membrane with the Ca2+ ionophore A23187 or artificial redox systems.Conclusion. The obtained data indicate that a high-fat, high-carbohydrate diet leads to metabolic and hemorheological disorders that are typical of metabolic syndrome.
Highlights
Feeding the rats with a high-fat, high-carbohydrate diet for 12 weeks resulted in obesity, blood pressure (BP) elevation, hyperglycemia, impaired glucose tolerance, and dyslipidemia with pronounced triglyceridemia
Multidirectional changes in the membrane potential were observed in response to the stimulation of the KCa-channels in the erythrocyte membrane with the Ca2+ ionophore A23187 or artificial redox systems
The obtained data indicate that a high-fat, high-carbohydrate diet leads to metabolic and hemorheological disorders that are typical of metabolic syndrome
Summary
Бирулина Ю.Г.1, Иванов В.В.1, Буйко Е.Е.1, 2, Трубачева О.А.1, 3, Петрова И.В.1, Гречишникова А.Ю.1, Носарев А.В.1, Гусакова С.В.1. Цель – изучить воздействие высокожировой и высокоуглеводной диеты на эритроциты и тромбоциты крови крыс. Исследование выполнено на 23 самцах крыс линии Вистар, которые были разделены на контрольную и опытную группу. Крысы контрольной группы находились на стандартной диете. У животных опытной группы происходило увеличение количества лейкоцитов, главным образом, за счет гранулоцитов, повышение числа тромбоцитов и их коллаген-индуцированной агрегации. Количественные показатели клеток красной крови крыс опытной группы не отличались от контрольной группы. В ответ на стимуляцию КСа-каналов мембраны эритроцитов животных экспериментальной группы с помощью Са2+-ионофора А23187 или редокс-системы наблюдались разнонаправленные изменения мембранного потенциала. Полученные результаты свидетельствуют, что высокожировая и высокоуглеводная диета приводит к метаболическим и гемореологическим нарушениям, характерным для метаболического синдрома. Ключевые слова: высокожировая и высокоуглеводная диета, метаболический синдром, ожирение, дислипидемия, клетки крови, КСа-каналы, агрегация. Исследование одобрено локальным этическим комитетом СибГМУ (заключение No 8201 от 27.03.2020)
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