Abstract
A reliable and sensitive technique had presented to monitor the content of long-chain fatty acids (LCFAs) in biological materials. The purpose of this study was to report a method for separation and quantification of LCFAs by capillary gas chromatography (GC) with better recovery. With this method, we detected some changes of LCFAs in the serum and liver of rat fed with high fat diet. The result showed that the levels of C22:0, C22:6, C26:0 of serum of the high fat group were higher than that of the control group (P<0.01); and the levels of C22:6, C26:0 of liver of the high fat group were higher than that of the control group (P<0.01), but the levels of C22:0 and C24:0 of liver of the high fat group were lower than that of the control group (P<0.01). It indicated that a high fat diet had an adverse effect upon normal metabolism.
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