Effects of a compound microbial agent and plants on soil properties, enzyme activities, and bacterial composition of Pisha sandstone.

Abstract

The objective of this study was to investigate the effects of the microbial agent on the improvement of Pisha sandstone soil and find out an effective measure for the control of soil erosion in the Pisha sandstone area. Pisha sandstone containing the microbial agent composed of Bacillus halotolerans P75, Sinorhizobium meliloti D10, Bacillus megaterium H3, Bacillus subtilis HB01, and organic substrate was utilized to be the soil matrix for pot experiment, and then alfalfa, ryegrass, and caragana were planted, respectively. Effects of the microbial agent plus plants on the soil properties of Pisha sandstone were evaluated, and the results showed that the microbial agent plus plants significantly increased the organic matter content, alkali hydrolyzed nitrogen content, available phosphorus content, available potassium content, invertase activity, and urease activity. Meanwhile, inoculation with the microbial agent significantly promoted the growth of alfalfa, ryegrass, and caragana and also influenced the number of soil bacteria and the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria, and others. However, the effects of the microbial agent plus different plants on the soil properties and bacterial composition of Pisha sandstone were different, while the growth of different plants showed differences, suggesting that there was a different interaction between microbes and different plants in Pisha sandstone soil. In conclusion, the microbial agent plus plants could improve Pisha sandstone soil which could provide some theoretical and experimental references for soil erosion control in the Pisha sandstone area.