Effects of a chromated-copper-arsenate wood preservative on the growth of a pentachlorophenol degrading bacterium

Abstract

A chromated-copper-arsenate (CCA) wood preservative was tested for toxic effects on the growth of a bacterial culture (Flavobactenum sp. ATCC 53874) capable of biodegrading pentachlorophenol, another wood preservation chemical. Both a commercially available CCA preparation and a laboratory-prepared CCA solution were tested. Each had an inhibitory effect on the growth of Flavobacterium at diluted CCA levels as low as 1.0×10−4 to 1.0×10−5% wt vol−1. The commercial formulation was generally more toxic. EC50 values calculated after 96 hr of incubation were 1.2 ×10−4% wt vol−1 for the commercial material (containing 0.15/0.097/0.14 μg mL−1 of Cr/Cu/As, respectively) and 3.8×10−4% for the laboratory solution (containing 0.51/0.31/0.49 μg mL−1 of Cr/Cu/As, respectively). CCA toxicity increased during the first 7 to 8 days and then slowly decreased for the balance of the 21 day incubation period. Biodegradation of pentachlorophenol residues in contaminated soil will be negatively affected by the presence of CCA as a co-contaminant.