Effects of 7,12-dimethylbenz(a)anthracene on growth and haematological parameters in Korean rockfish, Sebastes schlegeli (Hilgendorf)

Abstract

The objectives of the present experiment were to determine the effects on growth factors and some haematological parameters in Korean rockfish, Sebastes schlegeil, after dietary 7,12-dimethylbenz(a)anthracene (DMBA) exposure at 0.6, 1.2, 2.4 and 4.8 mg kg[superscript [-]1] diet for 8 weeks. The specific growth rate of the fish exposed to DMBA ([>/=]1.2 mg kg[superscript [-]1]) showed significantly lower performance than the control. Following 8 weeks of exposure, the DMBA-exposed groups (2.4 and 4.8 mg kg[superscript [-]1]) had a significantly higher mean hepatosomatic index. Condition factor of the fish exposed to DMBA did not show any statistically significant deviation from the control (P>0.05), although the mean values were somewhat lower than those of the control group. Observations on haematological parameters indicated DMBA treatment induced a severe anaemia. Total protein, albumin and total cholesterol concentrations decreased following exposure to DMBA at 2.4 and 4.8 mg kg[superscript [-]1]. In contrast, serum bilirubin and blood urea nitrogen in fish exposed to DMBA increased. Significant decreases in serum electrolytes, chloride, phosphorus, magnesium and calcium, and osmolality were observed in all DMBA-treatment groups. All DMBA-treatment groups showed a significantly higher activity of lactate dehydrogenase and alkaline phosphatase. Serum transaminase activity after the highest level of 4.8 mg kg[superscript [-]1] DMBA was significantly increased. The key finding from this study is that rockfish exposed to dietary DMBA at concentrations of 1.2 and 2.4 mg kg[superscript [-]1] diet are likely to experience adverse impacts in growth and haematological property respectively.