Abstract
Normal DNA Synthesis The DNA molecule is a double helix consisting of two complementary chains of nitrogenous bases connected by sugar-phosphate backbones [ 171. Complementarity is introduced by the rule that adenine must pair with thymine and guanine with cytosine. Fig. 1 illustrates the three general schemes by which such a molecule may duplicate [3]. It is possible to distinguish among these models of replication by giving the progeny and parental DNA different density labels and using the density gradient technique developed by Meselson, Stahl, and Vinograd [lo]. Meselson and Stahl [9] used 15N to label the DNA of E. coli and found that after the first division all of the DNA was l/2 labeled, and that after the second division l/2 the DNA was l/2 labeled and l/2 fully labeled. These results were P&RENT FIRST DIVISION SEiJND DI”ISION
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