Abstract
Objective To observe the effects of 4-phenylbutyrate (4-PBA) on the ischemia-reperfusion injury of rat skin flaps by inhibiting endoplasmic reticulum stress. Methods Seventy-five Wistar rats were used to create the abdominal island skin flaps. They were then randomly divided into three groups: control group, 4-PBA group and saline group (NC group). In NC and 4-PBA groups, a skin flap tissue sample was removed at the time the skin flap was created 0, 1, 6, 12 and 24 hours following ischemia reperfusion. In the control group, a skin flap tissue sample was removed at the time the skin flap was created 0, 1, 6, 12 and 24 hours following the surgical procedure. Pathological observation, detection of apoptosis by TUNEL method, RT-PCR detection of the expression level of GRP78 and CHOP mRNA, Western-blot and immunofluorescence staining for expression level of GRP78 and CHOP proteins were carried out. The correlation was analyzed. Results Histology showed that the tissue swelling degree, necrotic cells and inflammatory cells in NC group was more severe than 4-PBA group following 6 hours of ischemia and reperfusion. The number of TUNEL positive cells in 4-PBA group, compared with NC group, were reduced by 19.4% and 26.1%, respectively, following 6 and 24 hours of ischemia and reperfusion with significant difference (P<0.05). The levels of both mRNA and protein expression of GRP78 and CHOP were lower in 4-PBA group than NC group following 6, 12 and 24 hours of ischemia and reperfusion with significant difference (P<0.05). Conclusion 4-PBA reduces cell apoptosis in rat skin flaps following ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress-mediated apoptosis mechanism. It plays an important role in protecting rat skin flaps against ischemia reperfusion injury and apoptosis. Key words: Surgical flaps; Animal experimentation; Ischemia reperfusion injury; Endoplasmic reticulum stress; 4-phenylbutyrate
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
