Effects of 3-phenylamino-l,2-propanediol and its mono- and dioleylesters on the production of reactive oxygen metabolites in human polymorphonuclear leukocytes

Abstract

Human polymorphonuclear leukocytes (PMNL) were exposed to 3-phenylamino-l,2-propanediol (PAP) or to its monoor dioleylesters. The dioleylester of PAP (140 μM) slightly increased the production of reactive oxygen metabolites (ROM) from 8 ± 2 mV to 18 ± 3 mV, whereas PAP and its mono-oleylester were without any effect on ROM production in PMNL. None of the compounds were able to modulate formyl-Methionyl-Leucyl-Phenylalanineinduced production of ROM. Moreover, PAP did not have any effect on the production of ROM induced by phorbol myristate acetate (PMA). On the other hand, both monoand dioleylesters of PAP dose-dependently inhibited PMA-induced production of ROM. The dioleylester of PAP, at a concentration of 14 μM, inhibited PMA-induced production of ROM by 79%, whereas a 2.3 mM concentration of mono-oleylester of PAP was required for 65% inhibition of PMA-induced production of ROM. Moreover, both esters of PAP shifted the peak production of ROM by PMA to a later time-point. Our results suggest that monoand dioleylesters of PAP modulate the responses of PMNL upon stimulation with PMA, whereas PAP does not have any effect on PMA-induced ROM production in PMNL. © 1997 Elsevier Science Ireland Ltd.