Effects of 3D-printed bulking agent on microbial community succession and carbohydrate-active enzymes genes during swine manure composting

Abstract

The bulking agent plays an important role in aerobic composting, but their shape, porosity, and homogeneity need to be optimized. In the present work, a bulking agent with a uniform shape was prepared by 3D printing to explore its influence on physicochemical parameters, microbial community succession, and gene abundance of carbohydrate-active enzymes (CAZymes) in swine manure aerobic composting. The results showed that adding 3D-printed bulking agents can increase maximum temperature, prolong the thermophilic period, and improve the degradation rate of volatile solids, which was attributed to ameliorative air permeability by the porous 3D-printed bulking agent. The abundances of some pathogenic bacteria decreased and CAZymes genes increased respectively in response to the addition of the 3D-printed bulking agent, implying it has a certain positive effect on improving the safety of compost products and promoting the degradation of organic matter. In summary, the 3D-printed bulking agent has good application potential in laboratory-scale aerobic composting.