Effects of 3,4-dichloroaniline (3,4-DCA) and 4,4′-methylenedianiline (4,4′-MDA) on sex hormone regulation and reproduction of adult zebrafish (Danio rerio)

Abstract

3,4-dichloroaniline (3,4-DCA) and 4,4′-methylenedianiline (4,4′-MDA) have been widely used in manufacture of many industrial and consumer products, and hence often detected in aquatic environment. Reproductive toxicity of aniline and its derivatives in aquatic organisms has been suggested, however, knowledge on the endocrine disruption potentials and toxicological consequences of both anilines are not well understood, especially in fish. In this study, we aimed to understand the effects of 3,4-DCA and 4,4′-MDA on sex hormone regulation and reproduction of adult zebrafish (Danio rerio). Following 21 d exposure, significant decreases of the reproduction were observed at 0.38 mg/L 3,4-DCA, and 4.6 mg/L 4,4′-MDA. Moreover, plasma concentrations of testosterone (T) and 17β-estradiol (E2) level were significantly decreased in both male and female fish following the exposure. The sex hormone changes could be explained by the regulatory changes of the genes along the hypothalamic-pituitary-gonadal (HPG) axis, including significant down-regulation of steroidogenic acute regulatory protein (star) and cytochrome P450 family 19 subfamily A (cyp19a) genes in the gonad. Moreover, inhibition of gonadotropin hormone signaling and prostaglandin-endoperoxide synthase 2 (ptgs2) gene expression were observed, suggesting potential disruption of oocyte maturation and ovulation by the exposure. Our observations indicate that 3,4-DCA and 4,4′-MDA can impair reproduction of zebrafish potentially through disruption of steroid hormone synthesis and ovulation.