Effects of 25-hydroxycholecalciferol supplementation in maternal diets on reproductive performance and the expression of genes that regulate lactation in sows.

Abstract

One hundred Yorkshire×Landrace sows were randomly assigned to one of two dietary treatments (diet ND: 6,000IU vitamin D3 /d feed; diet 25-D: 200μg/day 25OHD3 feed). The experiment began on d 90 of gestation and continued until weaning on day 21 of lactation. In sows that received 25OHD3 , the growth rate of the piglets before weaning was significantly accelerated (0.266kg/day, p<.05). Sow serum was collected after weaning, and those in the 25OHD3 group were found to have significantly higher serum calcium (CA) and phosphorus (PI) levels (p<.05). Interestingly, the oestrus cycle of sows fed 25OHD3 was significantly shortened (p<.05), the oestrus time was concentrated on the fifth day after weaning, and the piglets were born with a higher degree of uniformity (p<.05). Colostrum was collected on the day of delivery, and the colostrum of sows fed 25OHD3 contained higher milk fat content than the control group (p<.05). 25OHD3 supplementation increased the mRNA and protein expression of INSIG1 and SREBP1, which regulate milk fat synthesis, in the mammary gland of lactating sows (p<.05). In conclusion, 25OHD3 supplementation in maternal diets improved reproductive performance, milk fat content and the mRNA and protein levels of genes regulating milk fat synthesis in lactating sows.