EFFECTS OF 2,3,5‐TRIIODOBENZOIC ACID ON THE STRUCTURE OF SOYBEAN LEAVES

Abstract

The effects of 2,3,5‐triiodobenzoic acid (TIBA) on soybean leaves (Glycine max. [L.] Merrill ‘Harosoy‘) include thickening with intensification of color and some raised intercostal regions, giving a wrinkled appearance. These effects are not restricted to early stages of leaf development but are pronounced during and after unfolding of the leaf. Proliferation of tracheary elements, increased procambial activity, and hypertrophy of bundle sheath extension cells occurred in the leaflet midvein of the youngest expanded leaf treated with 50 ppm or 100 ppm of TIBA. The youngest treated leaves exhibited differential growth rates and expansion within the palisade and spongy layers. Hypertrophy of spongy cells in these leaves occurred independently or simultaneously with elongation of the upper and lower palisade layers. The palisade and spongy tissues had undergone cell division and expansion at a greater pace than the epidermal layers. This, along with hypertrophy in the bundle sheath extension cells, would explain the wrinkled appearance of the lamina. The treated leaf became thicker than the control as a result of the increased number of cells in the spongy layer and elongation in the palisade layer. The observed aberrations in leaf structure suggest that TIBA interferes with some auxin‐translocating system within the plant.