Abstract
The diamondback moth, Plutella xylostella (Linnaeus), is one of the notorious pests causing substantial loses to many cruciferous vegetables across the nations. The effects of 60Co-γ radiation on physiology of P. xylostella were investigated and the results displayed that 200 Gy irradiation significantly alters the antioxidant enzyme regulation in six-day-old male pupae of P. xylostella. First, in our research, we detected Oxidase system and stress response mechanism of irradiated pupae, the results displayed that 200 Gy irradiation significantly alters the antioxidant enzyme regulation in six-day-old male pupae of P. xylostella. The levels of superoxide dismutase (SOD) and catalase (CAT) were increased significantly in contrast the level of peroxidase (POD) and glutathione S-transferase (GST) were decreased in 12–24 h post-treatment. The heat shock proteins (Hsps) gene expression level was significant increasing, maximum > 2-folds upregulation of genes were observed in peak. However, they also had a trend of gradual recovery with development. Second, we detected the testis lactate dehydrogenase (LDH) and acid phosphatase (ACP) activity found that in male adults testis they increased significantly than control during its development. Thus the present research investigation highlights that the 60Co-γ radiation treatments alters the physiological development of diamondback moth. The results showed that 200 Gy dosage resulted in stress damage to the body and reproductive system of the diamondback moth.
Highlights
The diamondback moth (DBM), P. xyllostella (L.) is one of the most critical notorious pests of cruciferous vegetables across the nations [1]
Effect of 60 Co-γ Radiation on the Antioxidant Enzymes of Pupae Irradiated with 200 Gy
Effect of 60Co-γ Radiation on the Antioxidant Enzymes of Pupae Irradiated with 200 Gy
Summary
The diamondback moth (DBM), P. xyllostella (L.) is one of the most critical notorious pests of cruciferous vegetables across the nations [1]. Insects are not immune to the ravages of reactive oxygen species (ROS), which are a by-product of oxidative metabolism in aerobic cells and are produced following exposure of cells and tissues to various stressors [11,12,13]. The endogenous enzymatic antioxidant system is important for protecting the organism against high levels of ROS This system is mainly composed of the enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione S-transferase (GST) [14,15,16,17]. Ionizing radiation produces ROS, which can induce the expression of HSP. P. xylostella was a substerilizing dose, inducing 26% and 36% sterilities respectively [26] This enabled further exploration of the effect of irradiation on testis. The research on sperm is being carried out in the laboratory but is not yet completed
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