Abstract
The aim of this study was to investigate effects of 1,25(OH) 2D 3 (calcitriol), 25OHD 3, and EB1089 on cell growth and on Vitamin D receptor (VDR) mRNA and 1α-hydroxylase (1α-OHase) mRNA expression in normal canine prostatic primary cultures. Canine prostatic epithelial cells were isolated, cultured, and treated with vehicle (ethanol), calcitriol, 25OHD 3, and EB1089 at 10 −9 and 10 −7 M. The VDR was present in epithelial and stromal cells of the canine prostate gland. 1,25(OH) 2D 3, 25OHD 3, and EB1089 inhibited epithelial cell growth at 10 −7 M compared to vehicle-treated controls [calcitriol ( P<0.01), EB1089 ( P<0.01), and 25OHD 3 ( P<0.05)]. Epithelial cells treated with calcitriol and EB1089 at 10 −7 M had slightly increased VDR mRNA expression (0.2–0.3-fold) at 6 and 12 h compared to controls. There was no difference in 1α-OHase mRNA expression in epithelial cells treated with these three compounds. 1,25(OH) 2D 3 and its analogs may be effective antiproliferative agents of epithelial cells in certain types of prostate cancer.
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