Abstract
In this study we have used for the first time the infrared spectroscopy as a tool to reveal the effects induced on a cell line, Jurkat T-lymphocytes, by ultrasound (US) used in therapy at 1 MHz frequency. We carried out the experiments using two different setups allowing to change the acoustic field parameters by varying the position of the sample with respect to the ultrasonic transducer and the sonication time with different duty cycles. The parameters of acoustic field measured by a hydrophone were compatible with the cavitation phenomenon in both setups. Genotoxic damage in exposed cells was ascertained by the cytokinesis-block micronucleus (CBMN) assay whilst cell structural changes due to sonication, were revealed by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, a non-destructive technique that allows, by the acquisition of IR spectra within a few minutes, one to obtain simultaneous information on all cellular macromolecules in a cell population. Infrared spectroscopy results highlight changes of functional groups typical of proteins, nucleic acids and lipids at 118 J/cm 2 energy dose, while the micronuclei test shows no toxic damage to the cellular samples; higher energy doses are necessary to reveal significant toxic effects together with structural changes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
