Effects and mechanism of selenium combined with vitamin B6 on the level of blood lipid in patients with hyperlipidemia

Abstract

Objective To study the effects of selenium combined with vitamin B6 on blood lipid and lipid metabolism in patients with hyperlipidemia, and to explore its regulatory mechanism. Methods A total of 100 patients with hyperlipidemia detected by physical examination during June 2017 to December 2017 were divided into control group and experimental group according to stratified-block random method, with 50 cases in each group. Patients in experimental group took the selenium (50 μg/d) enriched yeast vitamin E tablets and vitamin B6 tablets (10 mg/d). Patients in control group took vitamin E tablets with ordinary yeast. Both of them were treated for 30 days. The routine physical examination (including rounting blood test and blood biochemical examination) was performed on the 0th day and the 30th day, respectively. Serum level of selenium, vitamin B6, total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C) were measured. On the 30th day of the experiment, the levels of serum 3-hydroxyl-3-methylamyl two coenzyme A reductase (HMGR), liver lipase, lipoprotein lipase (LPL) and total lipase were detected in both groups. The mRNA level of silencing information regulator 1 (SIRT1), peroxisome proliferator-activated receptor α (PPARα) and sterol regulatory element binding protein-1c (SREBP-1c) in the whole blood were analyzed by real-time transcription polymerase chain reaction. Results The physical examination indexes of experimental group were not different from those in control group on the 0th day and the 30th day after the intervention (all P>0.05). Compared with the 0th day, the level of serum albumin in control group and experimental group on the 30th day were decreased (t=5.942, 6.162, all P 0.05). The serum level of total cholesterol and LDL-C on the 30th day in experimental group were lower than those of control group and the 0th day when compared with itself (t=2.960, 2.990, all P<0.05; t=2.190, 2.086, all P<0.05). On the 30th day, compared with control group, the serum level of HMGR was decreased but the liver lipase, LPL and total lipase levels were increased (t=5.839, -2.072, 3.115, -2.323, all P<0.05). The level of SIRT1 and PPARα mRNA in the whole blood of experimental group were higher than those of control group, but the level of SREBP-1c mRNA was lower than that of control group (t=6.295, 7.662, 6.857, all P<0.05). The serum level of selenium and vitamin B6 in experimental group were higher than those of control group at the 30th day and the 0th day when compared with itself (t=-2.606, -2.710, all P<0.05; t=-4.296, -3.690, all P<0.05). Conclusions Moderate supplementation of selenium enriched yeast combined with vitamin B6 can reduce the level of serum total cholesterol and LDL-C among people with hyperlipidemia. The mechanism may be caused by the regulation of the expression of key lipid metabolic regulators such as SIRT1, PPARα and SREBP-1c, as well as the secretion of lipid metabolism enzymes such as HMGR, liver lipase and LPL. Key words: Selenium combined with vitamin B6; Hyperlipidemia; Lipid metabolism