Effects and mechanism of juglone in combination with 5-FU on colon cancer CT-26 cells in vivo

Tianyu Liu,Yan Li,Na Li,J.Q Cheng,Z.T Chi,H.L Moffitt,J Zhang,I Kim

doi:10.1051/bioconf/20170801013

Abstract

The effects and mechanism of juglone alone or combined with 5-fluorouracil on colon cancer CT-26 cells were examined in vivo. Starting at 5 weeks of age, male Balb/c mice were transplanted the CT-26 cells and treated with juglone alone or combined with 5- fluorouracil. Observed the morphologic characteristics of tumor tissue by HE staining after treatment of 9 days. We detected the content of IL-2, VEGF and IFN-γ in serum and the protein expression of ki-67, E-cad and Bcl-2 in tumor tissue. The inhibition rate of animals given low-dose or high-dose juglone alone and combined with 5-fluorouracil is 31.73%, 40.38%, 56.73% and 75.01% respectively. Animals given juglone alone or combined with 5-fluorouracil could increase the content of IL-2 and IFN-γ in serum and the expression of E-cad and decrease the content of VEGF in serum and the expression of ki-67 and Bcl-2 significantly compared with those injected 2% ethanol NS (P <0.05 in each). The present data suggest that the juglone could reduce the risk of CT-26 cells metastasis and proliferation and enhance the function of the immune system. The possible anti-tumor mechanism of juglone is down-regulated expression of Bcl-2 to induce the apoptosis of tumor cells.

Highlights

Colon cancer as one of the malignant digestive tumor is a major global health problem[1]
These data showed that juglone combined with 5-fluorouracil was more cytotoxic than juglone alone and the effect of juglone combined with 5-fluorouracil (20mg/kg 5-FU) was better than 5-fluorouracil (20mg/kg 5-FU), indicating that juglone may have a good anti-cancer effect on CT-26 cells
By observing the morphological characteristics of CT-26 cells (Figure 1), we found both juglone and 5-fluorouracil could make tumor necrose obviously, such as some nucleus becoming smaller or cells becoming shrinkage even cytolysis

Summary

Introduction

Colon cancer as one of the malignant digestive tumor is a major global health problem[1]. 5 – fluorouracil, a metabolic type of antitumor drugs, is recognized in clinical to treat colon cancer and other digestive tract cancer [2]. Many data derived from laboratory studies have suggested that juglone has a good antitumor activity both in vitro or vivo. It has obvious inhibitory effect on the proliferation of Hela cells and esophageal cancer cells in vitro and breast cancer cells, stomach cancer cells and S180 solid cells in mice [5,6,7,8]. The effects and mechanism of juglone alone or combined with 5-fluorouracil on colon cancer CT-26 cells were examined in vivo. The effects of juglone and juglone combined with 5-fluorouracil on immune function in vivo is discussed

Methods

Results

Conclusion