Effects and mechanism of adiponectin in promoting adipose tissue wound healing of subcutaneous suture by inhibiting adipocyte apoptosis

Abstract

Objective To investigate relationship between adiponectin and adipocyte apoptosis during adipose tissue wound healing (ATWH),and clarify the role and its mechanism of adiponectin in subcutaneous suture to promote ATWH.Methods The pig models of ATWH were established to detect adiponectin expression of local adipose tissue by reverse transcriptase-polymerase chain reaction (RT-PCR),and apoptosis was examined by fluorescent labeling technique.3T3-L1 preadipocytes of mice were cultured in vitro.In experimental group 3T3-L1 preadipocytes were cultured with different concentrations of adiponectin,and apoptosis rate was tested by Annexin-V-FITC method.Results The expression of adiponectin in subcutaneous suture was significantly higher than that in control group (P ＜ 0.05).After 3 days and 5 days,the number of apoptotic cells was 29 ± 13 and 15 ± 8,respectively,statistically significantly less than that in the control group (69 ±25 and 23 ± 16) (P ＜0.05).With adiponectin concentration rising,the number of apoptotic cells was (26.42 ± 1.58) ％,(15.14 ± 1.91) ％,(7.04 ± 1.16) ％ and (5.05 ± 0.83) ％,significantly less than that in control group (33.18 ± 1.86) ％ (P ＜ 0.05).Conclusion Adiponectin plays a catalytic role in ATWH in the subcutaneous suture,and inhibiting adipocytes apoptosis is one of its mechanisms. Key words: Adiponectin ; Subcutaneous suture; Apoptosis ; Wound healing