Effectivity of various media for biomass and lipid production of mixed culture of Glagah in open pond

Abstract

Biomass and lipid are produced from microalgae which have been known as one of the sources for producing biodiesel. The usage of biodiesel has several advantages, there are as a renewable energy source, less toxic, and zero CO2. Single culture of microalgae often to produce low biomass, which is can be overcome by using mixed consortium culture, such as a mixed culture of Glagah. It is also needed to consider the medium that will be used since the nitrogen concentration has been the key role in microalgae growth. The aims of this study were to measure the biomass and lipid content on the mixed culture of Glagah which cultured in Bold's Basal Medium (BBM), Farmpion, dan Thoriq Eko Arif Medium (TEAM). The mixed culture of Glagah was cultured in 7 days by using an open pond system. The growth of microalgae as the cell density was measured by using spectrophotometry. The dry weight of microalgae was obtained as the biomass production. Therefore, the lipid content was obtained by using the Bligh and Dryer method. Lastly, the carbon concentration was counted by using gravimetry method. Respectively, the usage of TEAM, Farmpion medium, and BBM produced the cell density as 4.30×109 cell·L−1, 4.20×109 cell·L−1, dan 4.04×109 cell·L−1. on the biomass calculation, the mixed culture of Glagah was obtained 0.677×103 mg·L−1, 0.65×103 mg·L−1, dan 0.38×103 mg·L−1 by using BBM, TEAM, Farmpion medium respectively. On the other hand, the percentage of lipid which produced by the mixed culture of Glagah was 53%, 36%, and 27% per dry weight by using BBM, Farmpion medium, and TEAM. Bold's Basal Medium (BBM) had the highest biomass and lipid content, then followed by Thoriq Eko Arief Medium (TEAM) and Farmpion medium, respectively. Further research has to be done to optimize the production of the mixed culture of Glagah with a variety of media in a mass scale open pond system which supported by Environmental parameters such as pH, light intensity, and temperature.