Effectivity of quercetin as antiviral to dengue virus-2 strain New Guinea C in Huh 7-it 1 cell line

Abstract

The prevalence of dengue virus infection (DENV) in Indonesia is still high compared to other tropical countries in the world. Unfortunately, the specific of antiviral drug to DENV is not available yet. The pure compound such as quercetin revealed a good antiviral to DENV candidat. Quercetin is a plant-derived flavonoid that can be found in wide variety of fruits and vegetables. It has been proven that quercetin able to improve body performance and reduce the risk of infection as well as inhibit DENV replication in Vero cell. However, the research on human cell line is not yet conducted. Therefore, this research aims to determine the effectivity of quercetin as antiviral drug towards DENV-2 strain New Guinea C in human cell line Huh 7 it-1. We used Focus Forming Assay and MTT assay To determine the value of IC50 and CC50, respectively. The result of CC50 value was 217.113 μg/mL and IC50 value was 18.41 μg/mL, with the SI value of 11.8. From the results imply that quercetin has low toxicity with high effectivity. Thus, quercetin suggested a good candidate of antiviral drug against DENV-2 in future.