Effectivity of nanovaccine against tick-borne encephalitis

Abstract

The tubular immunostimulating complex (Tl-complex) is a new nanoparticulate antigen delivery system, which was developed to enhance immunogenicity of different subunit antigens within anti-infectious vaccines and increase their economic efficacy and safety. TI-complexes are self-organized from mixture of triterpene glycosides from Cucumaria japonica, cholesterol and monogalactosyldiacylglycerol (MGDG) from marine macrophytes. MGDG plays role of lipid matrix for subunit protein antigen interrupted in TI-complexes. Microviscosity of MGDG was shown to influence the conformation and immunogenicity of protein antigen. Present work was aimed to study adjuvant effect of Tl-complexes on immunogenicity of chimeric protein antigen based on E protein domain III of tick-borne encephalitis (TBE) virus and OmpF porin of Yersinia pseudotuberculosis depending on MGDGs isolated from different marine algae and seagrass. It was shown that TI-complex including MGDG from Ulva lactuca was most effective. Immunization by chimeric antigen incorporated in TI-complexes provided a high level of animal protection in experimental infection with TBE. Thus, the proposed construction is promising for the development of vaccines against TBE.