EFFECTIVENESS OF PROPOLIS WITH ARTEMISININ COMBINATION THERAPY IN TREATING MICE INFECTED WITH PLASMODIUM BERGHEI

Nurindah Saloka Trisnaningrum,Hendri Astuty

doi:10.22159/ijap.2019.v11s1.012

Nurindah Saloka Trisnaningrum, Hendri Astuty

Open Access

https://doi.org/10.22159/ijap.2019.v11s1.012

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Abstract

Objective: This study aimed to ascertain the effectiveness of combination treatment with propolis and artemisinin-based combination therapy (ACT)in avoiding further resistance to ACT.Methods: A total of 35 mice were injected with Plasmodium berghei and divided into six equal groups: No treatment (negative control), ACT alone(positive control), 75-mg propolis/kg body weight (BW), 150-mg propolis/kg BW, ACT with 75-mg propolis/kg BW, and ACT with 150-mg propolis/kg BW. After 7 days of therapy, parasite density was calculated using a thin blood smear.Results: Parasite density significantly declined after combination treatment with ACT and 150-mg propolis/kg BW.Conclusion: Therapy with propolis alone showed no inhibitory effect on parasites, although its 150-mg/kg-BW dose was effective as an ACT adjuvantmalaria therapy in mice.

Highlights

Malaria is a common disease in many parts of the world
The decrease in parasitemia levels (PL) was studied in mice using a combination of peg earth and chloroquine; the results showed no significant decrease in parasitemia after therapy [5]
This study aimed to develop antimalarial therapy using a combination of artemisinin-based combination therapy (ACT) and propolis, which is found in Indonesia

Summary

Introduction

Malaria is a common disease in many parts of the world. In 2015, the number of malaria cases reached 214 million worldwide, with 438,000 related deaths. Africa has the highest prevalence of malaria (88%) followed by Southeast Asia (10%) and the Eastern Mediterranean region (2%). In 2014, 30,000,000 cases of high transmission malaria with >1 case per 1000 population and 36,500,000 cases of the cases of low transmission malaria were reported in Indonesia. Despite this high number of cases, the global malaria burden is decreasing; the malaria incidence rate declined to 37% in 2015 from that in 2000 [1,2]. Resistance to artemisinin, sulfadoxine/pyrimethamine, mefloquine, chloroquine, and several other drugs is common in most parts of Africa and throughout most of Southeast Asia [3]. Combination therapies are generally administered to improve the efficacy of resistant drugs

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Conclusion