Abstract
SignificanceEmploying mechanistically distinct therapies like Photodynamic Therapy (PDT) can be a promising strategy to overcome the multidrug- resistance in breast cancer resulting in the killing of chemotherapeutic drug-resistant cancer cells. ApproachIn this study, Pheophorbide a (Pheo-a), is being used as a potential photosensitizer to induce cell death in Doxorubicin (DOX) resistant breast cancer MCF-7 cells. Efficacy of Pheo-a mediated PDT against MCF-7/DOX cancer cells was evaluated by MTT proliferation assay and analyzing cellular morphological changes, live dead cell death assay. ResultsAll experiments were performed thrice in triplicate, and the results obtained were analysed for statistical significance. Results showed that the Pheo-a can induce photodynamic cell death in resistant cancer cells treated with a 2.5 μM concentration of Pheo-a followed by irradiation with a 660 nm red laser at a light dose of 10 J/cm2. ConclusionsThus, the results from this study suggest the utilization of chlorophyll-based PSs as a rational therapeutic intervention for the eradication of chemo drug-resistance in cancers.
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