Abstract
Oily bilge water (OBW) is complex wastewater generated from ship operation. In the present study, the application of ozonation pretreatment before the sequential batch reactor (SBR) for removing organics and polyhydroxyalkanoates (PHA) production from complex OBW were investigated. OBW was subjected to lab-scale ozone pretreatment under the various experimental conditions such as a specific reaction time (0 min–120 min), inlet ozone dose (0.5 g/L–2.5 g/L) and solution pH (4–8). Pretreatment of OBW by ozone improved biodegradability index (BDI) from 0.36 to 0.52. The GC–MS analysis revealed that the BDI enhancement was due to the formation of water-soluble carboxylic groups after 75 min of ozonation operated at solution pH 6 and 2 g/ L of O3 dose. The CODs removal efficiency was 68 % and 92 % for SBR and ozonized SBR respectively. Also, the biopolymer production rate was increased by 4.5 folds for ozonized SBR as the bioconversion of carboxylic compounds by using a mixed bacterial consortium (MBC) was stress-free. The functional groups and thermal property of the produced PHA were tested by various analytical techniques. These results confirmed that the bioconversion of ozonized OBW into valuable biological polyesters using MBC.
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