Effectiveness of multiple culture of arbuscular mycorrhizal fungi (AMF) from the rhizosphere of cocoa on host plant Pueraria javanica

Abstract

The utilization of single Arbuscular Mycorrhizal Fungi (AMF) is constrained by the propagation of the single spore and usually depends on the host plant. This study aims to examine the effectiveness of single culture propagation of several genera of AMF from the rhizosphere of cocoa and the concentration of NPK on the host Pueraria javanica. The study was designed with a completely randomized design with two factors. The first factor was the genus FMA which consisted of Acaulospora sp1 (M1), Acaulospora sp2 (M2), Glomus sp1 (M3), Glomus sp2 (M4), Glomus sp3 (M5). The second factor was that the concentration of liquid fertilizer was five levels: control (P0), 0.5g/l water (P1), 1.0g/l water (P2), 1.5g/l water (P3), 2.0g/l water (P4), and repeated four times. Parameters observed: root infection, spore number, main stem length, root length. The study showed that the highest number of spores resulted from the interaction of Acaulospora sp1 with 0.5g/l NPK. The most extended stem and root lengths were produced at 1.0g/l NPK with Acaulospora sp2, 27.40 cm, and 2.0 g NPK fertilizer with Acaulospora sp2, which had a root length of 8.8 cm. The highest colonization rate was found in plants not given NPK and infected with Glomus sp1. This study showed that the Pueraria Javanica plant is a suitable plant to be used as a host plant for the development of spore types Acaulospora and Glomous in single culture.