EFFECTIVENESS OF MELATONIN AND RESVERATROL COMBINED ADMINISTRATION IN ROUND-THE-CLOCK ILLUMINATION WITH HIGH-CALORIE CARBOHYDRATE-LIPID DIET

Abstract

The purpose of this experiment was to investigate the combined effect of exogenous melatonin and resveratrol on the serum melatonin content in rats exposed to 30 day round-the-clock lighting (RCL) and kept on a high-calorie carbohydrate-lipid diet (HCCLD, 20% fructose solution, and standard chow). The combination of exogenous melatonin and resveratrol was administered for 60 days to 35 white male rats. Exogenous melatonin and resveratrol were administered daily during the last 30 days of the experiment intragastrically in a dose of 5 mg/kg (both). The findings obtained have revealed the combined effect of RCL and HCCLD reduced the serum melatonin content to 7.1±0.7 pg/ml that is 77.7% (p<0.001) lower than the control values (31.8±2.5 pg/ml). Applying exogenous melatonin restored the concentration of this hormone in the blood serum to normal values, 28.4±1.6 pg/ml. Resveratrol administration increased the melatonin content to 13.6±1.0 pg/ml, i.e. by 91.5% (p<0.001). The combined administration of exogenous melatonin and resveratrol increased the concentration of melatonin in the blood serum to 32.3±2.0 pg/ml. It can be concluded that the combined effect of exogenous melatonin and resveratrol under RCL and HCCLD is more effective in correcting hypomelatoninemia compared to the effect of the compounds used separately.