Abstract
Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations
Highlights
Widodo WT (2020) Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus
Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera
Staphylococcus aureus is a gram positive coccus. This bacterium is often found as a normal germ flora in humans
Summary
Efektivitas Ekstrak Etanol digunakan metode difusi cakram kertas. Konsentrasi yang digunakan mulai dari 20%, 40%, 60%, 80% dan 100% dengan kontrol positif Erytromisin dan kontrol negatif aquades. Uji ekstrak etanol daun lidah biaya (Aloe vera) dalam menghambat bateri Staphylococcus aureus menghasilkan zona hambat pada konsentrasi 60%, 80% dan 100% dengan rerata diameter sebesar 6,94 mm; 6,22 mm dan 9,5 mm. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak etanol daun lidah buaya (Aloe vera) dapat menghambat Staphylococcus aureus dalam konsentrasi tinggi. Kandungan antibakteri lidah buaya (Aloe vera ) dapat berpotensi sebagai bahan obat untuk menyembuhkan infeksi bakteri Staphylococcus aureus. Berdasarkan uraian di atas peneliti tertarik melakukan penelitian tentang uji ekstrak etanol daun lidah buaya (Aloe vera) dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Bahan yang digu- nakan dalam penelitian ini yaitu etanol 70%, lidah buaya, NB, Nutrient agar, spirtus, dan bakteri Staphylococcus aureus. Pengujian daya hambat ekstrak etanol daun lidah buaya (Aloe vera) dilakukan dengan cara menyiapkan media NA dengan menimbang sebanyak 2,6 gram dalam 130 ml aquades. Diinkubasi pada suhu 37oC selama 24 jam lalu diamati dan diukur zona hambat yang terbentuk
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