Abstract
In this study, an attempt was made to investigate <i>in vitro</i> morphogenetic competence of three shrub species from the <i>Thymelaeaceae</i> family. The studied plant material originated from Russia, Greece and China, and the effectiveness of <i>in vitro</i> shoot formation and rhizogenesis of <i>Daphne caucasica</i>, <i>D. jasminea</i>, and <i>D. tangutica</i> was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm<sup>-3</sup> 2iP, 0.1 mg dm<sup>-3</sup> NAA, and 0.65 g dm<sup>-3</sup> calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B<sub>5</sub> vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in <i>D. tangutica</i>, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to <i>in vivo</i> conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.
Highlights
Thymelaeaceae is a family of flowering plants of cosmopolitan distribution, distinguished in the eighteenth century by Antoine Laurent de Jussieu, which includes genera representing mostly shrubs or trees, and considerably less frequently vines and herbaceous plants
Shoot explants were placed on medium consisting of macroelements from woody plant medium (WPM) (Lloyd and McCown, 1981), a mixture of micronutrients and vitamins, either from MS or B5 medium (Murashige and Skoog, 1962; Gamborg et al 1968), supplemented with 20 g×dm-3 sucrose, 0.6 g×dm-3 activated charcoal, 0.65 g×dm-3 calcium gluconate, 1.0 mg×dm-3 2-isopentenyladenine (2iP), 0.1 mg×dm-3 1-naphthaleneacetic acid (NAA), and solidified with 8 g×dm-3 Difco Bacto agar
During two successive 28-day-long passages on modified WPM medium containing 2 % sucrose, 1.0 mg×dm-3 2iP, 0.1 mg×dm-3 NAA, and 0.8% agar, shoot cultures were vital and produced adventitious buds, which showed a satisfactory rate of Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants 23 development (Fig. 1A-C)
Summary
Thymelaeaceae is a family of flowering plants of cosmopolitan distribution, distinguished in the eighteenth century by Antoine Laurent de Jussieu, which includes genera representing mostly shrubs or trees, and considerably less frequently vines and herbaceous plants. Daphne species (vanderBank , 2002; Herber , 2003). Such species are exploited in a diverse manner, for example in Japan and in Nepal particular species from the genus (D. bhoula, D. papyracea) have been used in writing paper production because of their exceptionally long fibres of secondary phloem. Species and cultivars belonging to this genus are frequently used as valuable ornamental plants which are more and more willingly used in European green areas due to attractive foliage as well as the tint of flowers and fruits. All parts of those excellent plants, and especially fruits, may be poisonous to mammals (Reichholf and Steinbach , 1995; Brickell and White , 2000; Latocha , 2006; Seneta and Dolatowski , 2009)
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